Objective:
- Follow the Zymo Research Quick-DNA/RNA MiniPrep Plus Kit protocol and homogenization here
Summary:
- Extractions:
- 10 samples on 20200910
- QC checklist:
- 10 total samples
Extraction and QC checklist:
| Experiment | Sample Identifiers | Extraction and QC | |||||
|---|---|---|---|---|---|---|---|
| Date fixed | Experiment day | Treatment | Geoduck ID | Extraction Date | Qubit completed (Y/N) | Gel completed (Y/N) | TapeStation completed (Y/N) |
| 20190730 | pre | Amb | 15 | 20200910 | Y | Y | Y |
| 20190730 | pre | Elev | 27 | 20200910 | Y | Y | Y |
| 20190731 | 7 | AHA | 439 | 20200910 | Y | Y | Y |
| 20190731 | 7 | AHS | 414 | 20200910 | Y | Y | Y |
| 20190807 | 14 | AHM | 754 | 20200910 | Y | Y | Y |
| 20190807 | 14 | EHM | 726 | 20200910 | Y | Y | Y |
| 20190814 | 21 | AHAA | 1651 | 20200910 | Y | Y | Y |
| 20190814 | 21 | AHMA | 1590 | 20200910 | Y | Y | Y |
| 20190814 | 21 | EHAA | 1609 | 20200910 | Y | Y | Y |
| 20190814 | 21 | EHMA | 1584 | 20200910 | Y | Y | Y |
Qubit Results
| Sample | DNA (ng/uL) | RNA (ng/uL) | ||
|---|---|---|---|---|
| RUN 1 | RUN 2 | RUN 1 | RUN 2 | |
| Standard 1 | 180.49 | 178.44 | 393.30 | 391.94 |
| Standard 2 | 20925.86 | 20696.05 | 9453.00 | 9438.61 |
| 15 | 23.4 | 23.6 | 68.2 | 67.8 |
| 27 | 38.2 | 38.4 | 159 | 155 |
| 439 | 31.2 | 31.4 | 82.6 | 81.4 |
| 414 | 52.2 | 52.4 | 118 | 114 |
| 754 | 17.3 | 17.3 | 34.6 | 33.2 |
| 726 | 48.6 | 48.4 | 137 | 136 |
| 1651 | 5.64 | 5.56 | 14.4 | 13.8 |
| 1590 | 60.4 | 60.8 | 144 | 143 |
| 1609 | 70.0 | 69.0 | NA | NA |
| 1584 | 57.6 | 57.8 | 210 | 210 |
Gel Electrophoresis
TapeStation Results
Run 1 link here