Objective and summary:
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Library prep fo 5 F1 Juvenile Bay scallops for lcWGS
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target this week to complete 25 library preps as 12 pH8, 12 pH 7.5 and one duplicate to submit for expedited seq lane (pre paid)
Library prep
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Protocol: Ran the modified Baym protocol shared by Nina T. - reviw notebook post of the protocol here
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I targeted a 2.5 ng/ul DNA concentration in 200 ul
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Samples:
| ID | Treatment | mean DNA | index primers |
|---|---|---|---|
| 3 | 8_A | 185 | H503 ; H710 |
| 53 | 8_B | 208 | H503 ; H705 |
| 101 | 8_C | 152 | H503 ; H706 |
| 201 | 7.5_A | 103 | H503 ; H707 |
| 251 | 7.5_A | 228 | H503 ; H711 |
- NOTE: used the 24 sample index primers, 4.4 ul from each index pair
Qubit Results
| Sample | DNA (ng/uL) | |
|---|---|---|
| RUN 1 | RUN 2 | |
| S1 | 99.71 | 98.10 |
| S2 | 12775.08 | 12775.08 |
| 3 | 8.61 | 8.60 |
| 53 | 11.7 | 11.7 |
| 101 | 16.6 | 16.6 |
| 201 | 18.7 | 19.1 |
| 251 | 16.5 | 16.8 |