Samuel J Gurr, PhD

Lab Notebook

..of an invertebrate enthusiast with a backbone

I am an advocate for open science and strive to dissolve inaccessible barriers. Public repositories (such as GitHub!) advance science, disseminating knowledge transparently and reproducibly. Interested in expanding upon this thread in my current and future career endevours. Please reach out if you have any suggestions or just want to talk science, happy to chat!

What to find here:

  • SOPs
  • bioinformatics roadblocks and rabbitholes
  • ideas and hypothesis
  • logged benchwork and other data

Posts - Page 8 of 17

Argopecten Qubit 20220131

  • 1 min read

Objective and summary:

  • Complete extraction and qubit quantification using non-target adult scallop samples

    • 30 mg and 90 mg of tissue
    • 3 hr Proteinase K incubation at 120 RPM and 55C - vortexed every hour
    • doubled the volume of TL Buffer, HBC buffer and 100% ethanol for the 60 mg samples (did not double proteinase K)

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