Objective(s):
- Prepare geoduck metabolomic samples for analysis following the follwoing links:
- Six samples were prepped on 8/11/20 for 1 animal and 4 animals in triplicate view table here - today I will prep three additional samples with 7 animals each final table at the end of this post
Extraction Protocol, important links (MSDS info), etc. link here
Notes during today’s extraction
- I initially targetted 10 animals in triplicate, however this is reduced to 7 animals per sample. Our whole geoduck have a broad size variation and some individuals are quite large. These samples are very concentrated and completely fill the glass dounce during homogenization.
- Notes on the protocol IMPORTANT!!
- 5 ml tube used for the initial weight measurements (vial, vial + buffer, vial + buffer + geoduck)
- Transfer from tube->dounce was modified to reduce potential error and contact with the buffer. use 1 ml pipette to transfer buffer and use a pipette tip to carefully transfer animals to the dounce.
Summary
Table for 20200813
| Sample # | Geoduck ID | Number animals | Vial (g) | Vial + Extraction buffer (g) | Vial + Extraction buffer + geoduck sample(s) (g) | Vial labels | | — | — | - | — | — | — | — | | 7 | 291,300,304,316 | 7 | 3.03 | 3.74 | 5.51 | 7, 7-A, 7-B | | 8 | 296,312,318 | 7 | 3.01 | 3.87 | 4.92 | 8, 8-A, 8-B | | 9 | 190,196,200 | 7 | 3.02 | 3.87 | 4.69 | 9, 9-A, 9-B |
Final table - all samples prepared to send to Rutgers (20200811 and 20200813)
| Date completed | Sample # | Geoduck ID | Treatment/Day | Number animals | Vial (g) | Vial + Extraction buffer (g) | Vial + Extraction buffer + geoduck sample(s) (g) | Vial labels |
|---|---|---|---|---|---|---|---|---|
| 20200811 | 1 | 684 | AHA / Day 11 | 1 | 1.02 | 1.9 | 2.14 | 1, 1-A, 1-B |
| 20200811 | 2 | 652 | AHS / Day 11 | 1 | 1.03 | 1.83 | 1.86 | 2, 2-A, 2-B |
| 20200811 | 3 | 621 | AHM / Day 11 | 1 | 1.04 | 1.88 | 1.95 | 3, 3-A, 3-B |
| 20200811 | 4 | 638,624 | AHM / Day 11 | 4 | 1.04 | 1.89 | 2.24 | 4, 4-A, 4-B |
| 20200811 | 5 | 612,632 | EHM / Day 11 | 4 | 1.03 | 1.89 | 2.38 | 5, 5-A, 5-B |
| 20200811 | 6 | 659,666 | EHS / Day 11 | 4 | 1.02 | 2.02 | 2.46 | 6, 6-A, 6-B |
| 20200813 | 7 | 291,300,304,316 | EHA / Day 4 | 7 | 3.03 | 3.74 | 5.51 | 7, 7-A, 7-B |
| 20200813 | 8 | 296,312,318 | AHA / Day 4 | 7 | 3.01 | 3.87 | 4.92 | 8, 8-A, 8-B |
| 20200813 | 9 | 190,196,200 | AHM / Day 4 | 7 | 3.02 | 3.87 | 4.69 | 9, 9-A, 9-B |
NOTE: under ‘Vial labels’ the #-A #-B are autosampler vials (each with 100 ul) and the # is a 1.5 ml tube with the remaining sample