20200817 & 20200818 Juvenile Panopea generosa DNA/RNA extractions
Objective and summary:
-
Follow the Zymo Research Quick-DNA/RNA MiniPrep Plus Kit protocol and homogenization here for 16 samples
-
Summary:
- Extractions
- 6 samples on 20200817
- all stored for QC on 20200818
- 10 samples on 20200818
- QC checklist:
- Qubit == all 16 samples (from 0818 and 0817)
- Gel electrophoresis == NA, gel did not take due to an error. Need to complete this for all 16 later
- Tape Station == completed for 12 samples. Four samples saved for a later date
Extraction and QC checklist:
| Experiment | Sample Identifiers | Extraction and QC | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Date fixed | Experiment day | Treatment | Geoduck ID | Storage Box # | Position | Extraction Date | Qubit completed (Y/N) | Gel completed (Y/N) | TapeStation completed (Y/N) |
| 20190807 | 14 | AHA | 814 | 16 | D6 | 20200817 | Y | N | Y |
| 20190807 | 14 | AHM | 729 | 16 | D4 | 20200817 | Y | N | Y |
| 20190807 | 14 | AHS | 771 | 10 | D4 | 20200817 | Y | N | Y |
| 20190807 | 14 | EHA | 822 | 17 | A7 | 20200817 | Y | N | Y |
| 20190807 | 14 | EHM | 750 | 10 | A3 | 20200817 | Y | N | Y |
| 20190807 | 14 | EHS | 785 | 10 | I8 | 20200817 | Y | N | Y |
| 20190807 | 14 | AHA | 849 | 16 | E5 | 20200818 | Y | N | Y |
| 20190807 | 14 | AHA | 859 | 16 | G7 | 20200818 | Y | N | Y |
| 20190807 | 14 | AHM | 737 | 16 | I5 | 20200818 | Y | N | Y |
| 20190807 | 14 | AHM | 735 | 17 | D6 | 20200818 | Y | N | Y |
| 20190807 | 14 | AHS | 797 | 16 | A1 | 20200818 | Y | N | Y |
| 20190807 | 14 | AHS | 777 | 19 | I1 | 20200818 | Y | N | Y |
| 20190807 | 14 | EHA | 825 | 17 | G8 | 20200818 | Y | N | N |
| 20190807 | 14 | EHA | 829 | 19 | B3 | 20200818 | Y | N | N |
| 20190807 | 14 | EHM | 717 | 16 | I8 | 20200818 | Y | N | N |
| 20190807 | 14 | EHS | 801 | 10 | E1 | 20200818 | Y | N | N |
Qubit Results
| Sample | DNA (ng/uL) | RNA (ng/uL) | ||
|---|---|---|---|---|
| RUN 1 | RUN 2 | RUN 1 | RUN 2 | |
| Standard 1 | 174.58 | 176.74 | 383.85 | 381.97 |
| Standard 2 | 17076.05 | 18736.28 | 9257.83 | 9255.68 |
| 814 | 11.7 | 10.6 | 31.6 | 31.0 |
| 729 | 44.4 | 39.0 | 90.6 | 88.4 |
| 771 | 9.12 | 8.44 | 11.2 | 14.2 |
| 822 | 72.8 | 65.6 | 192.0 | 192 |
| 750 | 16.8 | 15.2 | 28.2 | 28.4 |
| 785 | 7.10 | 6.40 | 23.2 | 23.4 |
| 849 | 19.6 | 18.8 | 56.2 | 55.4 |
| 859 | 43.2 | 39.0 | 56.2 | 84.0 |
| 737 | 20.2 | 18.7 | 12.4 | 12.4 |
| 735 | 22.8 | 21.4 | 53.6 | 53.0 |
| 797 | 23.6 | 21.6 | 53.6 | 484 |
| 777 | 39.2 | 35.4 | 59.6 | 59.8 |
| 825 | 14.4 | 13.0 | 14.4 | 14.2 |
| 829 | 70.6 | 65.8 | 236 | 232 |
| 717 | 96.8 | 88.4 | 176 | 177 |
| 801 | 102 | 94.4 | 110 | 109 |
TapeStation Results
Run 1 link here
Run 2 link here
