20200825 Juvenile Panopea generosa DNA/RNA extractions

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Objective and summary:

• Follow the Zymo Research Quick-DNA/RNA MiniPrep Plus Kit protocol and homogenization here for 16 samples

Summary:

• Extractions:
  • 10 samples on 20200825
• QC checklist:
  • Qubit == all 10 samples
  • Gel electrophoresis
    • all 10 sampels from 20200825
    • 1 sample from 20200818 (801)
    • all 6 samples from 20200817 (814,729,771,822,750,785)
  • Tape Station == 24 total samples

Extraction and QC checklist:

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Experiment			Sample Identifiers			Extraction and QC
Date fixed	Experiment day	Treatment	Geoduck ID	Storage Box #	Position	Extraction Date	Qubit completed (Y/N)	Gel completed (Y/N)	TapeStation completed (Y/N)
20190807	14	EHS	783	16	I1	20200825	Y	Y	Y
20190814	21	AHAA	1645	16	G4	20200825	Y	Y	Y
20190814	21	AHAA	1600	19	A6	20200825	Y	Y	Y
20190814	21	AHAM	1518	20	H1	20200825	Y	Y	Y
20190814	21	AHMA	1549	10	E8	20200825	Y	Y	Y
20190814	21	AHMA	1593	19	A9	20200825	Y	Y	Y
20190814	21	AHMM	1500	17	H5	20200825	Y	Y	Y
20190814	21	AHMM	1471	6	H7	20200825	Y	Y	Y
20190814	21	AHSM	1531	10	I4	20200825	Y	Y	Y
20190814	21	EHAA	1638	10	B6	20200825	Y	Y	Y

Qubit Results

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Sample	DNA (ng/uL)		RNA (ng/uL)
	RUN 1	RUN 2	RUN 1	RUN 2
Standard 1	165.46	164.89	390.21	392.26
Standard 2	17346.41	17274.20	9078.95	9082.1
783	9.98	10.0	10.6	10.6
1645	41.2	41.2	91.0	88.6
1600	7.80	7.80	NA	NA
1518	11.2	11.1	NA	NA
1549	57.4	57.0	131	130
1593	31.8	31.6	52.6	53.0
1500	38.0	37.8	98.8	98.6
1471	35.4	35.2	72.4	72.8
1531	66.6	66.4	161	159
1638	116	116	200	199

Gel Electrophoresis

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TapeStation Results

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Run 1 link here

Run 2 link here