20200827 Juvenile Panopea generosa DNA/RNA extractions

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Objective and summary:

• Follow the Zymo Research Quick-DNA/RNA MiniPrep Plus Kit protocol and homogenization here

Summary:

• Extractions:
  • 10 samples on 20200827
• QC checklist:
  • 20 total samples
    • 10 from today 20200827
    • all 10 from 20200826

Extraction and QC checklist:

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Experiment			Sample Identifiers			Extraction and QC
Date fixed	Experiment day	Treatment	Geoduck ID	Storage Box #	Position	Extraction Date	Qubit completed (Y/N)	Gel completed (Y/N)	TapeStation completed (Y/N)
20190814	21	AHMM	1464	17	G9	20200827	Y	Y	Y
20190814	21	AHSA	1578	21	C2	20200827	Y	Y	Y
20190814	21	AHSM	1537	18	D4	20200827	Y	Y	Y
20190814	21	EHAA	1647	19	F2	20200827	Y	Y	Y
20190814	21	EHAM	1521	10	D5	20200827	Y	Y	Y
20190814	21	EHAM	1489	9	C5	20200827	Y	Y	Y
20190814	21	EHMA	1582	8	H9	20200827	Y	Y	Y
20190814	21	EHMM	1459	11	A1	20200827	Y	Y	Y
20190814	21	EHSA	1576	11	G4	20200827	Y	Y	Y
20190814	21	EHSA	1567	9	G7	20200827	Y	Y	Y

Qubit Results

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Sample	DNA (ng/uL)		RNA (ng/uL)
	RUN 1	RUN 2	RUN 1	RUN 2
Standard 1	184.01	187.41	387.18	390.40
Standard 2	19955.45	20183.50	9330.25	9366.45
1464	18.0	18.0	46.8	46.4
1578	26.0	26.0	56.0	55.0
1537	35.8	35.0	162	164
1647	50.4	49.6	102	101
1521	14.4	14.3	42.6	42.4
1489	28.6	28.4	34.2	33.6
1582	20.6	20.2	21.8	20.8
1459	17.0	16.8	24.8	23.8
1576	10.5	10.5	NA	NA
1567	105	10.3	176	176
29	30.6	29.6	93.0	92.8
10	40.0	39.8	184	182
462	19.5	19.6	41.1	40.4
343	50.6	50.8	90.0	89.2
374	36.0	34.6	70.2	68.6
430	74.4	73.2	135	132
346	81.8	80.8	168	164
350	NA	NA	NA	NA
389	62.0	62.2	136	135
833	46.0	45.6	104	103

Gel Electrophoresis

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TapeStation Results

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Run 1 link here

Run 2 link here