20200827 Juvenile Panopea generosa DNA/RNA extractions
Objective:
- Follow the Zymo Research Quick-DNA/RNA MiniPrep Plus Kit protocol and homogenization here
Summary:
- Extractions:
- 10 samples on 20200903
- Also… 10 samples were extracted on 20200902 - aliquots were stored at -80 (RNA) and -20 (DNA) for QC today
- QC checklist:
- 20 total samples
- 10 from today 20200903
- all 10 from 20200902
- 20 total samples
Extraction and QC checklist:
| Experiment | Sample Identifiers | Extraction and QC | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Date fixed | Experiment day | Treatment | Geoduck ID | Storage Box # | Position | Extraction Date | Qubit completed (Y/N) | Gel completed (Y/N) | TapeStation completed (Y/N) |
| 20190731 | 7 | AHM | 362 | 12 | G4 | 20200902 | Y | Y | Y |
| 20190731 | 7 | AHS | 393 | 13 | B7 | 20200902 | Y | Y | Y |
| 20190731 | 7 | EHA | 426 | 14 | F3 | 20200902 | Y | Y | Y |
| 20190731 | 7 | EHM | 326 | 5 | E4 | 20200902 | Y | Y | Y |
| 20190731 | 7 | EHS | 385 | 14 | C6 | 20200902 | Y | Y | Y |
| 20190731 | 7 | EHS | 370 | 15 | I4 | 20200902 | Y | Y | Y |
| 20190731 | 7 | EHS | 409 | 5 | E3 | 20200902 | Y | Y | Y |
| 20190807 | 14 | AHA | 819 | 19 | B1 | 20200902 | Y | Y | Y |
| 20190807 | 14 | AHM | 757 | 17 | I8 | 20200902 | Y | Y | Y |
| 20190807 | 14 | AHS | 774 | 20 | F5 | 20200902 | Y | Y | Y |
| 20190814 | 21 | AHAA | 1641 | 18 | C2 | 20200903 | Y | Y | Y |
| 20190814 | 21 | AHAM | 1480 | 6 | I2 | 20200903 | Y | Y | Y |
| 20190814 | 21 | AHMA | 1630 | 10 | G9 | 20200903 | Y | Y | Y |
| 20190814 | 21 | AHMA | 1545 | 6 | D2 | 20200903 | Y | Y | Y |
| 20190814 | 21 | AHSA | 1623 | 6 | F5 | 20200903 | Y | Y | Y |
| 20190814 | 21 | EHAA | 1611 | 11 | G8 | 20200903 | Y | Y | Y |
| 20190814 | 21 | EHAM | 1462 | 17 | F3 | 20200903 | Y | Y | Y |
| 20190814 | 21 | EHMA | 1557 | 20 | D8 | 20200903 | Y | Y | Y |
| 20190814 | 21 | EHMM | 1455 | 11 | C5 | 20200903 | Y | Y | Y |
| 20190814 | 21 | EHSA | 1572 | 17 | F2 | 20200903 | Y | Y | Y |
Qubit Results
| Sample | DNA (ng/uL) | RNA (ng/uL) | ||
|---|---|---|---|---|
| RUN 1 | RUN 2 | RUN 1 | RUN 2 | |
| Standard 1 | 190.84 | 189.48 | 374.47 | 380.08 |
| Standard 2 | 19825.28 | 20872.99 | 7511.27 | 7580.82 |
| 362 | 55.2 | 53.8 | 230 | 230 |
| 393 | 74.0 | 70.8 | 240 | 238 |
| 426 | 8.88 | 8.54 | 21.8 | 20.6 |
| 326 | 53.0 | 50.4 | 175 | 172 |
| 385 | 45.8 | 43.8 | 91.8 | 90.8 |
| 370 | 21.4 | 20.4 | 58.6 | 57.4 |
| 409 | 77.8 | 74.2 | 171 | 168 |
| 819 | 22.8 | 21.8 | 23.0 | 22.0 |
| 757 | 60.8 | 57.8 | 151 | 149 |
| 774 | 22.4 | 21.4 | 31.0 | 29.8 |
| 1641 | 16.4 | 15.4 | 64.6 | 63.6 |
| 1480 | 48.2 | 46.0 | 130 | 128 |
| 1630 | 36.8 | 35.0 | 70.0 | 68.6 |
| 1545 | 82.6 | 78.8 | 404 | 398 |
| 1623 | 27.0 | 25.8 | 75.2 | 74.2 |
| 1611 | 40.0 | 38.0 | 122 | 119 |
| 1462 | 19.6 | 18.7 | 42.8 | 41.6 |
| 1557 | 39.6 | 37.6 | 75.4 | 74.0 |
| 1455 | 102 | 97.6 | 290 | 286 |
| 1572 | 75.4 | 73.0 | 222 | 220 |
Gel Electrophoresis
TapeStation Results
Run 1 link here
Run 2 link here