Objective:

• Follow the Zymo Research Quick-DNA/RNA MiniPrep Plus Kit protocol and homogenization here

Summary:

• Extractions:
  • 10 samples on 20200917
• QC checklist:
  • 20 total samples
    • 10 from 20200916
    • 10 from 20200917

Extraction and QC checklist:

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Experiment			Sample Identifiers	Extraction and QC
Date fixed	Experiment day	Treatment	Geoduck ID	Extraction Date	Qubit completed (Y/N)	Gel completed (Y/N)	TapeStation completed (Y/N)
20190807	14	EHA	841	20200916	Y	Y	Y
20190807	14	EHS	766	20200916	Y	Y	Y
20190814	21	AHAA	1603	20200916	Y	Y	Y
20190814	21	AHMM	1506	20200916	Y	Y	Y
20190814	21	EHAA	1482	20200916	Y	Y	Y
20190814	21	EHAM	1515	20200916	Y	Y	Y
20190814	21	EHMA	1627	20200916	Y	Y	Y
20190814	21	EHMM	1495	20200916	Y	Y	Y
20190814	21	EHSM	1539	20200916	Y	Y	Y
20190807	14	AHM	763	20200916	Y	Y	Y
20190730	pre	Elev	1	20200917	Y	Y	Y
20190807	14	EHM	741	20200917	Y	Y	Y
20190814	21	AHAM	1440	20200917	Y	Y	Y
20190814	21	AHMM	1504	20200917	Y	Y	Y
20190814	21	EHMA	1554	20200917	Y	Y	Y
20190814	21	EHMM	1498	20200917	Y	Y	Y
20190814	21	EHMM	1491	20200917	Y	Y	Y
20190814	21	EHSA	1597	20200917	Y	Y	Y
20190814	21	EHSM	1527	20200917	Y	Y	Y
20190814	21	EHSM	1474	20200917	Y	Y	Y

Qubit Results

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Sample	DNA (ng/uL)		RNA (ng/uL)
	RUN 1	RUN 2	RUN 1	RUN 2
Standard 1	190.55	188.56	401.31	403.81
Standard 2	21142.07	21016.37	9414.18	9448.73
841	59.8	59.4	149	147
766	17.3	17.0	35.8	35.6
1603	13.9	13.9	14.8	14.6
1506	45.6	46.2	99.4	98.4
1482	70.6	71.6	169	169
1515	50.2	50.2	125	124
1627	23.8	24.2	35.4	34.6
1495	8.12	8.40	17.8	16.6
1539	135	136	282	278
763	36.2	36.4	38.4	37.4
1	23.4	23.8	40.1	38.2
741	63.4	62.8	101	99.8
1440	53.6	54.2	126	123
1504	62.4	62.6	129	126
1554	44.2	43.6	156	153
1498	44.8	44.6	192	187
1491	10.7	10.8	35.8	34.6
1597	34.0	34.4	64.2	62.4
1527	72.2	71.2	228	224
1474	29.6	29.8	19.8	19.4

Gel Electrophoresis

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TapeStation Results

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Run 1 link here

Run 2 link here