Objective:

• Follow the Zymo Research Quick-DNA/RNA MiniPrep Plus Kit protocol and homogenization here

Summary:

• Extractions:
  • 6 samples on 20200923
• QC checklist:
  • 16 total samples
    • 10 SAMPLES EXTRACTED ON 20200918
    • 6 from 20200923

Extraction and QC checklist:

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Experiment			Sample Identifiers	Extraction and QC
Date fixed	Experiment day	Treatment	Geoduck ID	Extraction Date	Qubit completed (Y/N)	Gel completed (Y/N)	TapeStation completed (Y/N)
20190731	7	AHA	453	20200918	Y	Y	Y
20190731	7	AHM	334	20200918	Y	Y	Y
20190731	7	AHS	381	20200918	Y	Y	Y
20190731	7	EHA	441	20200918	Y	Y	Y
20190807	14	EHS	807	20200918	Y	Y	Y
20190814	21	AHAM	1452	20200918	Y	Y	Y
20190814	21	AHAM	1485	20200918	Y	Y	Y
20190814	21	AHSA	1560	20200918	Y	Y	Y
20190814	21	EHMA	1621	20200918	Y	Y	Y
20190807	21	EHSM	1543	20200918	Y	Y	Y
20190730	pre	Amb	7	20200923	Y	Y	Y
20190731	7	AHA	422	20200923	Y	Y	Y
20190731	7	EHA	434	20200923	Y	Y	Y
20190731	7	EHS	405	20200923	Y	Y	Y
20190814	21	EHSA	1632	20200923	Y	Y	Y
20190814	21	EHSM	1510	20200923	Y	Y	Y

Qubit Results

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Sample	DNA (ng/uL)		RNA (ng/uL)
	RUN 1	RUN 2	RUN 1	RUN 2
Standard 1	185.28	185.39	404.85	402.05
Standard 2	20753.71	20217.46	8349.49	8305.15
453	80.2	80.2	157	155
334	42.4	40.0	115	114
381	38.6	37.8	97.8	98.0
441	79.8	72.2	126	127
807	56.2	55.6	85.2	86.8
1452	38.2	38.4	108	110
1485	19.1	19.2	20.2	21.0
1560	52.6	53.2	260	262
1621	105	106	410	414
1543	67.6	68.2	196	199
7	29.0	29.8	68.0	68.8
422	43.2	43.4	131	131
434	68.8	68.4	114	116
405	50.4	50.6	91.4	92.8
1632	16.4	16.9	NA	NA
1510	70.4	71.6	174	177

Gel Electrophoresis

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TapeStation Results

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