Objective and summary:
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RNA extractions of juvenile A. irradians from OA challenge
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scallops extracted today were fixed in RNAlater alongside a physiological suite on 10/27/21
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NOTE 1: I am targetting 10 samples per treatment (8 and 7.5) on each date (10/27/21 and 2/28/22) for TagSeq. On 20220406 there were 4 samples with low yield, two from pH 8 and two from pH 7.5. I ran additional samples here with hope to get higher yield.
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NOTE 2: 4 extractions were completed for Molly’s Solidissima samples (surf clams) and are labelled as ‘M1, M2,.. etc’.
Qubit Results
| Sample | DNA (ng/uL) | |
|---|---|---|
| RUN 1 | RUN 2 | |
| Standard | 59.98 | 60.15 |
| Standard | 1142.68 | 1156.69 |
| M 1 | 87.4 | 85.0 |
| M 2 | 93.0 | 90.0 |
| M 3 | 4.45 | 4.36 |
| M 5 | 79.0 | 77.0 |
| 8 A 3 | 48.3 | 46.9 |
| 8 D 3 | 45.1 | 44.8 |
| 7.5 C 3 | 26.8 | 27.0 |
| 7.5 D 3 | 26.2 | 26.7 |
‘8’ represents the low pCO2 treatment (higher pH) ‘7.5’ represents the high pCO2 treatment (low pH)
‘**’ represents samples with low ng/ul yield - additional samples from these treatments were repeated to achieve a total of 10 samples per treatment (8 and 7.5) for TagSeq