Objective and summary:

• DNA extractions of juvenile A. irradians from October 25th 2021; objective is to use these extractions for lcWGS sequencing for GWAS, SNP calling, etc. alonside the parentage of F0s extracted for the same seq

• an online checklist can be found [here]{https://docs.google.com/spreadsheets/d/1RHM3u-njPWcJWpEaOIGDrHUU3swhVPZvZRTKvVuSV1Q/edit?usp=drive_web&ouid=108372819286763574086}

Qubit Results (dsDNA BR assay kit)

• NOTE: has several issues running the standards for the dsDNA BR assay kit (was a little rusty I guess) I opened a fresh 500 assay kit to diagnose this error and concluded that the reagent (or dye) must be thawed nearly completely and perhaps vortexed/spun down to receive a workable sample w/o error downstream - in summary the ‘old’ kit opened in January 2021 should still work fine

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Sample	DNA (ng/uL)
	RUN 1	RUN 2
Standard	43.21
Standard	3137.81
1	164
2	227
51	116
52	276
101	152
102	223
151	244
152	127
201	103
202	298
251	228
252	299