Lab Notebook

..of an invertebrate enthusiast with a backbone

I am an advocate for open science and strive to dissolve inaccessible barriers. Public repositories (such as GitHub!) advance science, disseminating knowledge transparently and reproducibly. Interested in expanding upon this thread in my current and future career endevours. Please reach out if you have any suggestions or just want to talk science, happy to chat!

What to find here:

  • SOPs
  • bioinformatics roadblocks and rabbitholes
  • ideas and hypothesis
  • logged benchwork and other data

Posts - Page 16 of 17

NADH dehydrogenase Pgenerosa Identification

  • 2 min read

OBJECTIVE

The following protocol was completed with the geoduck genome in preparation for primer design and qPCR of a target protein NADH dehydrogenase (complex I) This step-by-step process can be used to verify the presence/absence of a particular target gene within an annotated genome without gene family identification. In this case.. a database of the geoduck genome was used with only ID identifiers for genes (no family-specific accession ID)

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Experiment Prep Point Whitney

  • 1 min read

read.me

The following is a summary of a trip to Jamestown Point Whitney Shellfish Hatchery in Brinnon, Washingston frmo 3/6/20 - 3/13/20.

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AOX Pgenerosa identification

  • 2 min read

OBJECTIVE

The following protocol was completed with the geoduck genome in preparation for primer design and qPCR of a target protein Alternative oxidase This step-by-step process can be used to verify the presence/absence of a particular target gene within an annotated genome without gene family identification. In this case.. a database of the geoduck genome was used with only ID identifiers for genes (no family-specific accession ID)

Read More